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ScienCell human cervical epithelial cells (hcerec
( A ) Representative images showing HPV16 pseudovirions-derived GFP signal in primary human cervical <t>epithelial</t> cells <t>(hCerEC)</t> and primary fallopian tube epithelial cells (FTEC) incubated with different concentrations (MOI = 0.1, 0.5, 1, and 5) of HPV16 pseudovirions (PsV). The infection efficiencies of HPV16 PsV in hCerEC and FTEC cells are presented as the ratio of GFP-positive cells. Scale bar: 200 µm. ( B ) Quantitative data showing the ratio of GFP-positive cells in hCerEC and FTEC cells under different concentrations of HPV PsV. Each bar represented the mean ± SEM ( n = 4 technical replicates). Data were analyzed for significance using the two-way ANOVA followed by the Tukey’s multiple comparisons post hoc test. A value of P < 0.05 was considered statistically significant. **** P < 0.0001; *** P < 0.001; ** P < 0.01; ns: not significant, between indicated two groups. Exact P values for all comparisons are presented with the corresponding source data, which is available online. ( C ) Representative images showing the presence of HPV16 PsV-derived GFP signal in hCerEC, FTEC, FT194, FT246, COV362, and OVSAHO cells incubated with of HPV16-Psv (MOI = 2.0) for 72 h. Scale bar: 100 µm. ( D ) Quantitative results showing the ratio of HPV16 PsV positive cells in hCerEC, FTEC, FT194, FT246, COV362, and OVSAHO cells. Each bar represented the mean ± SEM ( n = 4 technical replicates). Bars with different letters are significantly different from each other ( P < 0.05). Data were analyzed for significance using the one-way ANOVA followed by the Tukey’s post hoc test. A value of P < 0.05 was considered statistically significant. Exact P values when compared to FTECs: P = 0.0059 for hCerEc; P = 0.0526 for FT194; P = 0.9903 for FT246; P < 0.00011 for COV326; P < 0.0001 for OVSAHO. ( E ) Quantitative data showing mRNA levels of YAP1 and the putative HPV receptors ( ITGA6 , SDC1 , and EGFR ) in hCerEC, FTEC, Hela, and OVSAHO cells. Each bar represented the mean ± SEM ( n = 3 technical replicates). Bars with different letters are significantly different from each other ( P < 0.05). Data were analyzed for significance using the one-way ANOVA followed by the Tukey’s multiple comparisons post hoc test. A value of P < 0.05 was considered statistically significant. Exact P values for all comparisons are presented with corresponding source data, which is available online. ( F ) HPV16 PsV infected fallopian tube epithelial cells in vivo. HPV16 PsV (1.0 × 10 6 pfu/µl in 30 µl saline with Evans blue dye) was injected into the right uterus (near the oviduct) of C57BL/6 mice ( n = 5 technical replicates). The left uterus was injected with the same amount of saline with Evans blue dye and used as a negative control. The presence of HPV16 PsV in the oviducts and ovarian bursa was monitored by the blue color (left panel). Yellow arrow points to ovary and ovarian bursa. White arrow pints to uterus. Scale bar: 3.0 mm. Representative images showing the presence of HPV16 PsV-derived GFP signal in the epithelium of mouse oviduct. Nuclei were stained with DAPI (blue). Scale bar: 100 µm. .
Human Cervical Epithelial Cells (Hcerec, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nanjing KeyGen Biotech Co Ltd human cervical surface epithelial cell hcerepic
( A ) Representative images showing HPV16 pseudovirions-derived GFP signal in primary human cervical <t>epithelial</t> cells <t>(hCerEC)</t> and primary fallopian tube epithelial cells (FTEC) incubated with different concentrations (MOI = 0.1, 0.5, 1, and 5) of HPV16 pseudovirions (PsV). The infection efficiencies of HPV16 PsV in hCerEC and FTEC cells are presented as the ratio of GFP-positive cells. Scale bar: 200 µm. ( B ) Quantitative data showing the ratio of GFP-positive cells in hCerEC and FTEC cells under different concentrations of HPV PsV. Each bar represented the mean ± SEM ( n = 4 technical replicates). Data were analyzed for significance using the two-way ANOVA followed by the Tukey’s multiple comparisons post hoc test. A value of P < 0.05 was considered statistically significant. **** P < 0.0001; *** P < 0.001; ** P < 0.01; ns: not significant, between indicated two groups. Exact P values for all comparisons are presented with the corresponding source data, which is available online. ( C ) Representative images showing the presence of HPV16 PsV-derived GFP signal in hCerEC, FTEC, FT194, FT246, COV362, and OVSAHO cells incubated with of HPV16-Psv (MOI = 2.0) for 72 h. Scale bar: 100 µm. ( D ) Quantitative results showing the ratio of HPV16 PsV positive cells in hCerEC, FTEC, FT194, FT246, COV362, and OVSAHO cells. Each bar represented the mean ± SEM ( n = 4 technical replicates). Bars with different letters are significantly different from each other ( P < 0.05). Data were analyzed for significance using the one-way ANOVA followed by the Tukey’s post hoc test. A value of P < 0.05 was considered statistically significant. Exact P values when compared to FTECs: P = 0.0059 for hCerEc; P = 0.0526 for FT194; P = 0.9903 for FT246; P < 0.00011 for COV326; P < 0.0001 for OVSAHO. ( E ) Quantitative data showing mRNA levels of YAP1 and the putative HPV receptors ( ITGA6 , SDC1 , and EGFR ) in hCerEC, FTEC, Hela, and OVSAHO cells. Each bar represented the mean ± SEM ( n = 3 technical replicates). Bars with different letters are significantly different from each other ( P < 0.05). Data were analyzed for significance using the one-way ANOVA followed by the Tukey’s multiple comparisons post hoc test. A value of P < 0.05 was considered statistically significant. Exact P values for all comparisons are presented with corresponding source data, which is available online. ( F ) HPV16 PsV infected fallopian tube epithelial cells in vivo. HPV16 PsV (1.0 × 10 6 pfu/µl in 30 µl saline with Evans blue dye) was injected into the right uterus (near the oviduct) of C57BL/6 mice ( n = 5 technical replicates). The left uterus was injected with the same amount of saline with Evans blue dye and used as a negative control. The presence of HPV16 PsV in the oviducts and ovarian bursa was monitored by the blue color (left panel). Yellow arrow points to ovary and ovarian bursa. White arrow pints to uterus. Scale bar: 3.0 mm. Representative images showing the presence of HPV16 PsV-derived GFP signal in the epithelium of mouse oviduct. Nuclei were stained with DAPI (blue). Scale bar: 100 µm. .
Human Cervical Surface Epithelial Cell Hcerepic, supplied by Nanjing KeyGen Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cervical surface epithelial cell hcerepic - by Bioz Stars, 2026-04
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( A ) Representative images showing HPV16 pseudovirions-derived GFP signal in primary human cervical epithelial cells (hCerEC) and primary fallopian tube epithelial cells (FTEC) incubated with different concentrations (MOI = 0.1, 0.5, 1, and 5) of HPV16 pseudovirions (PsV). The infection efficiencies of HPV16 PsV in hCerEC and FTEC cells are presented as the ratio of GFP-positive cells. Scale bar: 200 µm. ( B ) Quantitative data showing the ratio of GFP-positive cells in hCerEC and FTEC cells under different concentrations of HPV PsV. Each bar represented the mean ± SEM ( n = 4 technical replicates). Data were analyzed for significance using the two-way ANOVA followed by the Tukey’s multiple comparisons post hoc test. A value of P < 0.05 was considered statistically significant. **** P < 0.0001; *** P < 0.001; ** P < 0.01; ns: not significant, between indicated two groups. Exact P values for all comparisons are presented with the corresponding source data, which is available online. ( C ) Representative images showing the presence of HPV16 PsV-derived GFP signal in hCerEC, FTEC, FT194, FT246, COV362, and OVSAHO cells incubated with of HPV16-Psv (MOI = 2.0) for 72 h. Scale bar: 100 µm. ( D ) Quantitative results showing the ratio of HPV16 PsV positive cells in hCerEC, FTEC, FT194, FT246, COV362, and OVSAHO cells. Each bar represented the mean ± SEM ( n = 4 technical replicates). Bars with different letters are significantly different from each other ( P < 0.05). Data were analyzed for significance using the one-way ANOVA followed by the Tukey’s post hoc test. A value of P < 0.05 was considered statistically significant. Exact P values when compared to FTECs: P = 0.0059 for hCerEc; P = 0.0526 for FT194; P = 0.9903 for FT246; P < 0.00011 for COV326; P < 0.0001 for OVSAHO. ( E ) Quantitative data showing mRNA levels of YAP1 and the putative HPV receptors ( ITGA6 , SDC1 , and EGFR ) in hCerEC, FTEC, Hela, and OVSAHO cells. Each bar represented the mean ± SEM ( n = 3 technical replicates). Bars with different letters are significantly different from each other ( P < 0.05). Data were analyzed for significance using the one-way ANOVA followed by the Tukey’s multiple comparisons post hoc test. A value of P < 0.05 was considered statistically significant. Exact P values for all comparisons are presented with corresponding source data, which is available online. ( F ) HPV16 PsV infected fallopian tube epithelial cells in vivo. HPV16 PsV (1.0 × 10 6 pfu/µl in 30 µl saline with Evans blue dye) was injected into the right uterus (near the oviduct) of C57BL/6 mice ( n = 5 technical replicates). The left uterus was injected with the same amount of saline with Evans blue dye and used as a negative control. The presence of HPV16 PsV in the oviducts and ovarian bursa was monitored by the blue color (left panel). Yellow arrow points to ovary and ovarian bursa. White arrow pints to uterus. Scale bar: 3.0 mm. Representative images showing the presence of HPV16 PsV-derived GFP signal in the epithelium of mouse oviduct. Nuclei were stained with DAPI (blue). Scale bar: 100 µm. .

Journal: EMBO Reports

Article Title: HPV-YAP1 oncogenic alliance drives malignant transformation of fallopian tube epithelial cells

doi: 10.1038/s44319-024-00233-3

Figure Lengend Snippet: ( A ) Representative images showing HPV16 pseudovirions-derived GFP signal in primary human cervical epithelial cells (hCerEC) and primary fallopian tube epithelial cells (FTEC) incubated with different concentrations (MOI = 0.1, 0.5, 1, and 5) of HPV16 pseudovirions (PsV). The infection efficiencies of HPV16 PsV in hCerEC and FTEC cells are presented as the ratio of GFP-positive cells. Scale bar: 200 µm. ( B ) Quantitative data showing the ratio of GFP-positive cells in hCerEC and FTEC cells under different concentrations of HPV PsV. Each bar represented the mean ± SEM ( n = 4 technical replicates). Data were analyzed for significance using the two-way ANOVA followed by the Tukey’s multiple comparisons post hoc test. A value of P < 0.05 was considered statistically significant. **** P < 0.0001; *** P < 0.001; ** P < 0.01; ns: not significant, between indicated two groups. Exact P values for all comparisons are presented with the corresponding source data, which is available online. ( C ) Representative images showing the presence of HPV16 PsV-derived GFP signal in hCerEC, FTEC, FT194, FT246, COV362, and OVSAHO cells incubated with of HPV16-Psv (MOI = 2.0) for 72 h. Scale bar: 100 µm. ( D ) Quantitative results showing the ratio of HPV16 PsV positive cells in hCerEC, FTEC, FT194, FT246, COV362, and OVSAHO cells. Each bar represented the mean ± SEM ( n = 4 technical replicates). Bars with different letters are significantly different from each other ( P < 0.05). Data were analyzed for significance using the one-way ANOVA followed by the Tukey’s post hoc test. A value of P < 0.05 was considered statistically significant. Exact P values when compared to FTECs: P = 0.0059 for hCerEc; P = 0.0526 for FT194; P = 0.9903 for FT246; P < 0.00011 for COV326; P < 0.0001 for OVSAHO. ( E ) Quantitative data showing mRNA levels of YAP1 and the putative HPV receptors ( ITGA6 , SDC1 , and EGFR ) in hCerEC, FTEC, Hela, and OVSAHO cells. Each bar represented the mean ± SEM ( n = 3 technical replicates). Bars with different letters are significantly different from each other ( P < 0.05). Data were analyzed for significance using the one-way ANOVA followed by the Tukey’s multiple comparisons post hoc test. A value of P < 0.05 was considered statistically significant. Exact P values for all comparisons are presented with corresponding source data, which is available online. ( F ) HPV16 PsV infected fallopian tube epithelial cells in vivo. HPV16 PsV (1.0 × 10 6 pfu/µl in 30 µl saline with Evans blue dye) was injected into the right uterus (near the oviduct) of C57BL/6 mice ( n = 5 technical replicates). The left uterus was injected with the same amount of saline with Evans blue dye and used as a negative control. The presence of HPV16 PsV in the oviducts and ovarian bursa was monitored by the blue color (left panel). Yellow arrow points to ovary and ovarian bursa. White arrow pints to uterus. Scale bar: 3.0 mm. Representative images showing the presence of HPV16 PsV-derived GFP signal in the epithelium of mouse oviduct. Nuclei were stained with DAPI (blue). Scale bar: 100 µm. .

Article Snippet: Primary human cervical epithelial cells (hCerEC) were purchased from ScienCell Research Laboratories, inc. (Carlsbad, CA).

Techniques: Derivative Assay, Incubation, Infection, In Vivo, Saline, Injection, Negative Control, Staining